In vitro Antibacterial Activity of Cichorium intybus against some Pathogenic Bacteria
Renu Verma
Department of Botany, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Ashish Rawat
Department of Botany, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Showkat Ahmad Ganie
Department of Botany, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Rajneesh K Agnihotri *
Department of Botany, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Rajendra Sharma
Department of Botany, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Surabhi Mahajan
Department of Microbiology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
Ankur Gupta
Department of Microbiology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra, India.
*Author to whom correspondence should be addressed.
Abstract
The root and leaf extracts of Cichorium intybus were investigated for antibacterial activity against gram negative pathogenic bacteria viz. Escherichia coli and Pseudomonas aeruginosa. The sensitivity was analyzed using Disk diffusion method at various concentrations where zone of inhibition was compared with the standard drug Cephotaxime. The extracts showed a wide spectrum of inhibition against the test pathogens. Methanolic extract of root and leaf proves to have the strongest antibacterial activity. Antibacterial activity of the test extracts at different inhibitory concentration varied significantly at 0.05 level of significance. The maximum activity was recorded at 200mg/ml concentration, the activity decreased with the decrease in the concentration of the extract. The present study reveals that the root and leaf extracts of Cichorium intybus would exert several beneficial effects by virtue of their antibacterial activity and could potentially be exploited as a source of natural antibacterial.
Keywords: Antibacterial activity, cephotaxime, Cichorium intybus, disk diffusion, sensitivity