In vitro Antioxidant, Cytotoxic and Antibacterial Screening of the leaves of Acridocarpous orientalis, Native to Sultanate of Oman
Ahlam Al-Abri
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
Hazaa Al-Maqbali
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
Afaf Weli *
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
Sadri Said
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
Amzad Hossain
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
Md. Sohail Akhtar
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Sultanate of Oman, Oman.
*Author to whom correspondence should be addressed.
Abstract
Aims: To determine antioxidant, cytotoxic and antimicrobial activities of organic extracts from leaves of Acridocarpous orientalis (qafas) from Sultanate of Oman.
Study Design: Brine shrimp test, DPPH assay and Disc diffusion method.
Place and Duration of Study: School of Pharmacy, University of Nizwa, Oman, December 2012.
Methodology: Hexane, chloroform, ethylacetate and hydroalcoholic extract were obtained by Kupchan’s partitioning of ethanol extract isolated from leaves of A. orientalis by maceration. Antioxidant activity was determined by free radical scavenging of (2,2-diphenyl-1-picrylhydrazyl, DPPH). The antimicrobial activity was checked using agar disc diffusion method against Gram-positive bacteria (Staphylococcus aureus) and two Gram-negative bacteria (Escherichia coli and Pseudomonus aeruginosa). Brine shrimp test was used to measure cytotoxic activity.
Results: All extracts demonstrated potential antioxidant activities, hydroalcoholic extract showed the strongest activity (RC50 = 6.11 μg/ml). The order of antioxidant activity was hydro alcohol > ethyl acetate > chloroform > hexane extract. Ethylacetate extract showed low activity against Pseudomonus aeruginosa. None of the extracts was found to be active against brine shrimp larvae.
Conclusion: A. orientalis could be considered as a good source of antioxidant compounds.
Keywords: Aridocarpous orientialis, qafas, DPPH, Oman, antimicrobial, antioxidant, cytotoxic