Free Radical Scavenging Activities of Nyctanthes arbor-tristis. L on Adjuvant Induced Arthritis in Rats
Smeera Thomas
Department of Biotechnology Engineering, Sahrdaya college of Engineering and Technology, Kodakara, Thrissur, Kerala, India.
J. Grace Nirmala
Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, (Karunya Institute of Technology and Sciences), Coimbatore, Tamil Nadu, India.
R. T. Narendhirakannan *
Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, (Karunya Institute of Technology and Sciences), Coimbatore, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: The present study was undertaken to explore in vivo antioxidant potential of ethanol extracts of Nyctanthes arbor-tristis leaf and stem in adjuvant induced arthritic rats.
Methodology: Arthritis induced rats were administered with extract of Nyctanthes arbor-tristis leaf and stem. (150 mg/kg body Weight/rat/day for 30 days.
Results: A significant decrease in paw edema was observed following oral administration of the leaf and stem extracts. A significant (p<0.05) increase in the level of tissue TBARS, GPx and catalase was seen in arthritis induced rats (group II) and NAT treated rats (group III and group IV) showed a significant decrease in lipid peroxides, GPx and catalase level to near normalcy. The activity of total tissue SOD was found significantly (p<0.05) low in arthritis induced rats (group II) while a substantial increase in the activity to near normal level was noticed in NAT administered rats. The alterations in hematological and other biochemical parameters were restored to near normal levels after a treatment period of 30 days. The structural changes of the tissues shows the therapeutic ability of Nyctanthes arbor-tristis stem and leaf in experimental animals which were further evidenced by histological observations made on the hind limb tissue.
Conclusion: As Nyctanthes arbor-tristis is of natural origin, it is a safe and effective intervention for free radical mediated diseases.
Keywords: Anti-inflammatory, Arthritis, In vivo antioxidant, lipid peroxidation, Superoxide dismutase.