Tyrosinase Inhibition, Antioxidant and Antibacterial Activity of Commercial Daisy Extract (Bellis perennis)
Enida Karić *
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Emir Horozić
Faculty of Technology, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina and Catholic School Centre "St. Francis", Klosterska 10, 75 000 Tuzla, Bosnia and Herzegovina.
Saša Pilipović
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina and Agency for Medicinal Products and Medical Devices of Bosnia and Herzegovina, Maršala Tita 9, 71 000 Sarajevo, Bosnia and Herzegovina.
Esmeralda Dautović
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Merima Ibišević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Amra Džambić
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Semir Čeliković
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
Arnela Halilčević
Faculty of Pharmacy, University of Tuzla, Urfeta Vejzagića 8, 75 000 Tuzla, Bosnia and Herzegovina.
*Author to whom correspondence should be addressed.
Abstract
Extracts obtained from plant material have widely applied in the chemical and pharmaceutical industries because they contain significant concentrations of biologically active substances. Commercial daisy extract (Bellis perennis) was used in this paper for in vitro testing of tyrosinase enzyme inhibition, and antioxidant and antimicrobial activity. Inhibition of the tyrosinase enzyme was determined by monitoring dopachrome formation at a wavelength of 492 nm. Antioxidant activity was tested using FRAP and DPPH methods, while antibacterial activity was tested by diffusion technique on reference strains from the ATCC collection. The results showed that daisy extract inhibits tyrosinase enzyme in a dose-dependent manner. The extract effectively neutralized DPPH radicals and also showed good reducing ability. Bacterial strains used for in vitro antimicrobial activity testing did not show sensitivity to the extract concentrations used in this study.
Keywords: Daisy extract, tyrosinase, FRAP, DPPH, antimicrobial activity