Discriminating Benign and Malignant Brain Tumors By Spectral Analysis of Cerebrospinal Fluid
V. Masilamani *
Masila Research Centre, Thiruppangili-620015, India.
R. Vidhya Shree
Sri Venkateswara Engineering College, Sriperumbudur-602117, India.
Ajay T. Prasad
Department of Neurosurgery, Madras Medical College, Chennai-600002, India.
Kannan Ramamurthy
National Centre for Ultrafast Processes, University of Madras, Chennai-600113, Tamil Nadu, India.
Chellappan Selvaraju
National Centre for Ultrafast Processes, University of Madras, Chennai-600113, Tamil Nadu, India.
Wafa Mohammed Alsaleh
College of Science and Health Professions, King Saud bin Abdulaziz University for Health Science, Saudi Arabia and Department of Physics, College of Science, Hofuf, Saudi Arabia.
Tahani A. Alrebdi
Department of Physics, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box-84428, Riyadh 11671, Saudi Arabia.
Trinka Vijmasi
Masila Research Centre, Thiruppangili-620015, India.
A. Thiruvalluvan
Department of Neurosurgery, Madras Medical College, Chennai-600002, India.
V. Chandramouleeswaran
Department of Neurosurgery, Madras Medical College, Chennai-600002, India.
*Author to whom correspondence should be addressed.
Abstract
The preliminary study is concerned with an innovative technique for the discrimination of malignant brain tumor from benign with reasonable sensitivity and specificity. The entire study is based on the relative fluorescence intensity and life-time of bio-fluorophores like tryptophan and tyrosine which are then validated as a set of ratio parameters. Resultantly, it was seen that malignancy was characterized by three-fold enhancement of tyrosine. The picosecond-time resolved decay time measurement was done, which exhibited two-fold decrease in decay time for malignant sample. The improved accessibility, with minimal invasiveness to the patient, and lower cost are encouraging aspects of this technique.
Keywords: Cerebrospinal fluid (CSF), fluorescence spectra, time resolved spectra, malignant brain tumor, benign brain tumor