The Assessment of the Allium sativum and Tamarix aphylla Comparative and Combined Antioxidant Potential
Shahid Ullah
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Fahim Ullah Khan *
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Laiq Zaman
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan and Department of Zoology, Kohat University of Science and Technology, Kohat-26000 Khyber Pakhtunkhwa, Pakistan.
Saira Abbas
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Mir Sadiq Shah
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Jamil Ur Rehman
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Autif Hussain Mangi
Institute of Biochemistry, University of Sindh, Jamshoro (76090), Pakistan.
Abdu Razzaq Khan
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Batab Khan
Department of Zoology, University of Science and Technology Bannu (28100), Kp, Pakistan.
Surayia Shahab Rani
Department of Pharmacy, University of Sargodha, Sargodha, Punjab (40100), Pakistan.
Sana Gul
Department of Chemistry, University of Science and Technology Bannu (28100), Kp, Pakistan.
Adnan khattak
Department of Botany, University of Science and Technology Bannu (28100), Kp, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Many diseases, including cancer and diabetes mellitus, are caused by reactive oxygen species (ROS). Allium sativum (Garlic) contains vitamins A, B, and C, as well as effective drugs like insulin, alliin, mineral salts, mucilages, allicin, and volatile oils. Garlic has antioxidant properties which showed a therapeutic effect on some cancer types. The overall goal of this study was to conduct pharmacological testing to assess the combined antioxidant abilities of Allium sativum (cloves) methanolic and Tamarix aphylla (leaves) extracts. The extract demonstrated garlic activities in a dose-dependent manner, with scavenging activity of 21, 32, and 39% at different concentrations of 100, 500, and 1000 µg/mL.
The antioxidant activity of Tamarix aphylla methanolic extract was 29, 37, and 62% using the DPPH free radical scavenging assay at different concentrations of 100, 500, and 1000 µg/mL. However, combining extracts revealed the greatest scavenging activity at various concentrations of 100, 500, and 1000 µg/mL. By using the DPPH free radical scavenging assay, the combined methanolic extract of Tamarix aphylla and Allium sativum demonstrated substantial antioxidant activity of 45, 65, and 75% at concentrations of 100, 500, and 1000 µg/mL. According to our findings, the combined therapy of Tamarix aphylla and Allium sativum significantly inhibited DPPH free radicals. Tamarix aphylla and Allium sativum combined therapy may play an important role in the inhibition of free radicals that cause cancer.
Keywords: Allium sativum, Tamarix aphylla and antioxidant activity