Spectrophotometric Assessment of Benzene 1,3,5-Triol in Pure Forms and in Bulk Sample by Diazotiization Coupling Reaction
Mohauman Mohammed Majeed Al-Rufaie *
Department of Chemistry, Faculty of Sciences, Kufa University, Najaf, Iraq.
Aymen Abdul Rasool Jawad
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kufa University, Najaf, Iraq.
Lamia Abdultef Risan Al-Iessa
Department of Chemistry, Faculty of Sciences, Kufa University, Najaf, Iraq.
*Author to whom correspondence should be addressed.
Abstract
The method was published for determining microgram quantities of benzene 1,3,5-triol (phloroglucinol) in aqueous solution and some bulk samples using an easy, quick, and effective spectrophotometric method. The method uses a diazotization as well as coupling process in basic medium to produce a bright yellow water-soluble dye from Phloroglucinol and diazotized 4-Methoxy aniline That is stable and also has a maximum absorption wavelength of 420 nm. With a molar absorptivity of 1.5989x 104l mol-1 cm-1 as well as index of Sand ell sensitivity 0.001 g.cm- 2, Beer's law is followed across a concentration range of (2-40) g.ml-1 of phloroglucinol.The ideal conditions for all color development are detailed, and even the suggested procedures for determining phloroglucinol in aqueous medium as well as some bulk sample have been successfully employed.
Keywords: Diazotization coupling, phloroglucinol, 4-Methoxy aniline, spectrophotometric assessment