Comparative Evaluation of Alpha-Amylase and Alpha- Glucosidase Inhibitory Potential of Aqueous Seed Extract of Trigonella Foenum-Graecum and Moringa Oleifera - An In vitro Study
N. Prithiksha
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India.
R. Gayathri *
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India.
V. Vishnu Priya
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India.
J. Selvaraj
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India.
S. Kavitha
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Trigonella foenum-graecum is one of the commonly used herbs in food. Moringa oleifera is a source of food, accommodation and conventional medicine for many peoples in the developing countriesThe seeds of both the plants were explored for antidiabetic potential.
Methods: The current work was designed to probe the in vitro anti diabetic potential of the aqueous seed elicit of trigonella foenum- graceus and Moringa oleifera using the enzymes alpha amylase and alpha glucosidase.Both the extracts were screened for their phytochemicals and antioxidant potential was also analysed.The data were examine statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance in conjunction with groups. The results with the p<0.05 level were considered to be statistically significant.
Result: Due to its chemical ingredient and active compounds like amino acids, alkaloids, flavonoids, it proceeds as a good antioxidant. Trigonella foenum-graecum seed extract exhibited comparatively higher antidiabetic potential with an IC50 of 300µg/ml than the Moringa oleifera extract. Results of the work designate that both extracts of the plant possessed by forbidding alpha amylase, alpha glucosidase show maximum inhibition. Hence concluded that, Trigonella foenum graecum leaves might be considered as herbal remedies for diabetes.
Conclusion: In vitro antioxidant and antidiabetic potential of seed elicit of Trigonella foenum-graecum and Moringa oleifera were analyzed and compared. This study shows that Trigonella foenum-graecum has higher antidiabetic potential than the Moringa oleifera extract.
Keywords: Alpha amylase, alpha glucosidase, fenugreek, Moringa oleifera, innovative technology, novel method