Boerhavia diffusa Mediated Selenium Nanoparticles and their Antioxidant and Anti-Inflammatory Activity
B. Sushma
Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
R. V. Geetha *
Department of Microbiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
S. Rajesh Kumar
Nanobiomedical Lab, Saveetha Dental College and Hospital Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Boerhavia diffusa belongs to species of flowering plant which is commonly called as *punarnava*. Its leaves are used as green leafy vegetables, with its high anti-inflammatory and expectorant properties selenium particles are synthesised. Selenium particles are used as nutritional supplements and an anti-microbial coating. Selenium (Se) was found to have anti-inflammatory properties, but its bioavailability and toxicity are limiting factors considerably.
Aim: To find the antioxidant and anti-inflammatory activities of Boerhavia diffusa mediated selenium nanoparticles
Materials and Methods: 1 gm of Boerhaavia diffusawas added to 100 ml of distilled water and allowed to boil for 10-15 minutes at 70 degree celsius and filtered. 60 ml of 20 milli molar sodium selenite is prepared in 250 ml of conical flask, 40 ml of filtered plant extract was mixed to it. The synthesized nanoparticle was preliminarily analysed using UV visible spectroscopy. Prior to the final step the nanoparticle solution was centrifuged at 8000 rpm to prepare nanoparticle pellet powder, it was dried in a hot air oven at 80 degree celsius. The dried powder was sent for characterisation. The leftover nanoparticle solution extract was sent to evaluate antioxidant activity through DPPH assay and anti-inflammatory activity through albumin denaturation assay.
Results: Selenium nanoparticles were achieved from boerhavia diffusa plant extract, which was evident with its significant colour change in the cultivation medium. The final centrifuge was found to have potent antioxidant and anti-inflammatory activities.
Conclusion: The selenium nanoparticles synthesized from leaf extract of Boerhaavia diffusa were evaluated for antioxidant and anti-inflammatory activities. This study concludes that extract bound selenium nanoparticles had potent antioxidant and anti-inflammatory activities. Therefore selenium nanoparticles may provide a great implication in the later studies for its potential activity.
Keywords: Boerhavia diffusa, selenium nanoparticles, characterization, antioxidant activity, anti-inflammatory activity, innovative technology