Studies on Chemotherapeutic Potential of Hydroethanolic Leaf Extract of Aegle marmelos on Human Lung Cancer Cell Lines
N. Bharath Kumar
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, Tamil Nadu, India.
G. Sridevi *
Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai-600077, Tamil Nadu, India.
R. Selvaraj
Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Velappanchavadi, Chennai-600077, Tamil Nadu, India.
S. Preetha
Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai-600077, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Lung cancer, one of the world’s leading causes of cancer death. There is an improvement in mortality by screening of lung cancer by low dose computed tomography. Long time ago, lung cancer was a reportable disease which now became a commonest cause of death due to cancer. Discovery of medicine for various diseases is still an ongoing process. A lot of diseases are still remaining a threat without any treatment or medicine. Herbal derived medicines are preferred because of their low side effects on patients.
Objective: To investigate the role of Aegle marmelos against human lung cancer cell lines (A549 cell line).
Materials and Methods: A549 cell lines were procured from NCCS (National center for cell sciences) Pune, India. It was cultured and viability of the cells before and after adding the extract was analysed using the MTT assay. mRNA amplification was done using real time PCR.Statistical analysis was done using ANOVA and dunken’s multiple test. Corresponding graphs are also plotted.
Results: The viability of the cells decreased from 100% to 50%. The mRNA expression of wnt and β-catenin decreased after the addition of the extract.
Conclusion: The study concluded that Aegle marmelos possesses a good, novel, innovative, anti- cancer activity against the lung cancer cell lines A549.
Keywords: Lung cancer, drug, innovative, Aegle marmelos, cell lines, cell viability