Preparation of Mouth Wash Using Musa sapientum Mediated Silver Nanoparticles and Its Antimicrobial Activity
A. Roshan
Department of Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India.
R. Priyadharshini *
Department of Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India.
S. Rajeshkumar
Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, Tamilnadu, India.
Palati Sinduja
Department of Pathology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Musa sapientum are primarily grown in tropical and subtropical countries, and are commonly used around the world for their nutritional values. Peels were examined for mineral, nutritional content. The product of the mineral content is that the concentration of potassium, calcium, magnesium, iron, manganese, bromine, and rubidium are high. M. sapientum pulp showed high antimicrobial activity against 13 gram-positive and gram-negative bacteria.
Materials and Methods: The plant extract was prepared. Silver nanoparticles were taken in the form of AgNO3. The plant extract was tested for its anti-inflammatory activity by protein denaturation assay. To assess the antimicrobial activity, the prepared extract was inoculated in different culture plates containing different microorganisms. The results obtained were collected and statistically analyzed in SPSS software and graphs were obtained.
Results: Analysis of antimicrobial activity shows The zone of inhibition in Mueller Hinton agar showed C. albicans zone of inhibition in the cultured plate with the diameter of 10 mm, 12 mm and 25 mm in 25 µL, 50 µL, and 100 µL respectively. S. mutans zone of inhibition in the cultured plate with the diameter of 15 mm, 18 mm, and 22 mm at 25 µL, 50 µL, and 100 µL respectively.
Conclusion: From the results obtained and analyzed we may conclude that silver nanoparticles synthesized using Musa sapientum extract can be used as a good anti-microbial agent. Its anti-microbial activity can be enhanced by increasing the concentration. Hence furt her advancements in this study could lead to producing and marketing natural product-based antimicrobial and anti-inflammatory agents.
Keywords: Anti-microbial, Musa sapientum, mouth wash, Muller Hinton agar, silver nanoparticles, green synthesis