Production of Protease Enzyme and Antimicrobial Activities from Marine Actinobacterium of Streptomyces SP
R. Ananya
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
P. Sivaperumal *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Anitha Roy
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
T. Lakshmi
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Actinobacteria are Gram-positive bacteria that have traits of both bacteria and fungus. They are found in both terrestrial and aquatic habitats and aid in the breakdown of deceased species' organic matter. Marine actinobacteria are unusual in that they have antibacterial, anticancer, antiviral, insecticidal, and enzyme inhibitory activities. The biggest genus of Actinobacteria is Streptomyces. They create more than two-thirds of all-natural antibiotics that are therapeutically relevant (e.g. Neomycin, Cypemycin, Grisemycin, Bottromycins, and Chloramphenicol). Alkaline proteases are generated by a variety of microorganisms, including yeast, bacteria, and plants. The process of destroying or suppressing disease-causing bacteria is referred to as antimicrobial activity. This is accomplished using a variety of antimicrobial potential as well as antiviral properties.
Aim: To find out the antimicrobial activity of protease enzymes produced by the Streptomyces sp of marine actinobacterium.
Materials and Methods: A sediment sample was collected and marine Actinobacteria were isolated in Kuster’s Agar Medium (KUA) and were identified based on their chemotaxonomical characteristics.
Results: Screening for protease was carried out to assess enzyme production and enzyme assay was carried out to assess the enzymatic activity. The effect and pH on enzyme production were observed. Then the antimicrobial potential of protease enzymes was tested against a few bacterial strains at optimum temperature and pH.
Conclusion: From the above study, we conclude that the protease enzyme obtained from Streptomyces shows a potential antimicrobial (antibacterial) effect.
Keywords: Protease enzyme, effect of pH, antibacterial potential, novel drug