Antioxidant and Cytotoxic Activity of Red Sandal Mediated Silver Nanoparticles: An In-vitro Study
M. Gajapriya
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, Tamil Nadu, India.
S. Balaji Ganesh *
Department of Periodontics, Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, Tamil Nadu, India.
S. Rajesh Kumar
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Nanoparticles, or matter ranging between 1 and 100 nm size, are of immense value in nowadays technological ventures, in all fields. Besides, if made of biodegradable materials, the preparation supports sustained drug release for a long time. Green chemistry is thus incorporated in nanotechnology. Red Sandalwood, commonly known as Raktachandan, is a tree that is native and endemic to India. The aim of this study is to evaluate the antioxidant and cytotoxic activity of red sandal mediated silver nanoparticles (AgNP).
Materials and Methods: This study involves the synthesis of silver nanoparticles (AgNP) incorporating red sandal. Here the preparation of red sandal incorporated AgNP was followed by a test for its antioxidant activity using DPPH assay by incubating the red sandal mediated silver nanoparticles solution for 30 mins, later the reduction in the quantity of DPPH free radicals was assessed dependent on the absorbance at 517 MN. BHT was employed as control and the percentage of inhibition was determined and cytotoxicity using brine shrimps. To that 10 nauplii were added slowly to each concentration. Then the red sandal mediated silver nanoparticles were added according to the concentration level. The plates were incubated for 24 hours. After 24hrs, noted for the number of live nauplii’s present.
Results: The red sandal mediated silver nanoparticle has considerably efficient antioxidant activity at high concentrations (50 μL). The red sandal mediated silver nanoparticle has considerably efficient cytotoxic activity at low concentrations (5 μL).
Conclusion: From the present study, it can be concluded that red sandal mediated silver nanoparticles have a considerably moderate cytotoxic and antioxidant activity at high concentrations.
Keywords: Red sandal, medicinal plant, antioxidant, cytotoxicity, green synthesis