Screening of Antibacterial Activity from Mangrove Plants (Rhizophora mucronata) Leaf Associated Actinobacterium of Streptomyces Species
John Francis
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India.
Lakshminarayanan Arivarasu *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India.
Pitchiah Sivaperumal
Department of Pharmacology, Marine Biomedical and Environmental Health Research Laboratory- Blue Laboratory, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai-600077, India.
Lakshmi Thangavelu
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai-600077, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Mangrove is a shrub or small tree that grows in coastal saline water mostly. The term is also used for tropical coastal vegetation consisting of special species. Mangroves occur worldwide mostly in the tropics and subtropics, mainly between latitudes 25° N and 25° S. The total mangrove forest area of the world was 137,800 square kilometres spanning 118 countries and territories
Materials and Methods: The fresh leaves of were collected from Pichavaram area, Tamilnadu. The leaves were washed thoroughly with tap water then surface sterilized with 1% of hydrogen peroxide. Then the sample was macerated in mortar and pestle.
Results and Discussion: The antibacterial activity of the rhizophora extract was analysed using two types of assays, namely disc diffusion method and Minimum Inhibitory Concentration (MIC) assay. The disc diffusion test was done for different concentrations for the selected clinical isolates (Klebsiella, streptococcus and vibrio ).
Conclusion: From this study ,we can conclude that has good antibacterial activity against these microorganisms (Klebsiella, streptococcus and vibrio) and further studies can be done in future.
Keywords: Mangrove, antibacterial, actinobacteria, xenobiotics, wetlands