Preparation of Ethanolic Extract of Cassia auriculata and Its Anti-Diabetic Activity
T. Srinivasa Surya Sitaram
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 77, Tamil Nadu, India.
Lakshminarayanan Arivarasu *
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 77, Tamil Nadu, India.
S. Rajeshkumar
Nanomedicine Laboratory, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai-77, Tamil Nadu, India.
Lakshmi Thangavelu
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 77, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Diabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history of ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Cassia auriculata is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Cassia auriculata leaves.
Methods: The methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu’s reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay.
Results: The extract shows very good anti-diabetic activity for the Cassia auriculata extract by using BSA and EAA Assay.
Conclusion: The leaf extracts of cassia auriculata exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of cassia auriculata have a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.
Keywords: Cassia auriculata, diabetic activities, ethanolic extract, α-amylase assay, α-glucosidase assay