Hepatoprotective Activity of Pongamia Pinnata Leaves on Antitubercular Drugs (Isoniazid & Rifampin) Induced Hepatotoxicity in Rats
Samba Siva Raju Derangula *
Department of Pharmacology, Bharath Institute of Higher Education & Research, Selaiyur, Chennai, Tamil Nadu, India.
N. S. Muthiah
Department of Pharmacology, Sree Balaji Medical College & Hospital, Chrompet, Chennai, Tamil Nadu, India.
H. S. Somashekar
Department of Pharmacology, Viswabharathi Medical College & Hospital, Kurnool, Andhra Pradesh, India.
E. Sukumar
Saveetha Institute of Medical & Technical Sciences, Chennai, Tamil Nadu, India.
K. Prabhu
Department of Anatomy, Sree Balaji Medical College & Hospital, Chrompet, Chennai, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Hepatoprotective effect of an ethanolic extract of Pongamia Pinnata on antitubercular drugs (isoniazid and rifampin) induced hepatotoxicity in rats.
Methods: The experiment used five groups of male wistar rats, each with six individuals. The two control groups were given gum acacia and a mixture of isoniazid and rifampin. The two test groups received 200 and 400 mg/kg of an ethanolic extract of the leaves of Pongamia Pinnata, respectively. The fifth group was given silymarin (50mg/kg, orally). The concentrations of serum Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline Phosphatase (ALP), tissue Malondialdehyde (MDA) & thiols were calculated. One-way ANOVA was used for statistical examination, monitored through Tukey's test.
Results: When rats were given a mixture of antitubercular drugs and a high dosage (400 mg/kg) of an ethanolic extract of Pongamia Pinnata, blood enzyme levels were lower than when they were given antitubercular drugs alone. The co-administration of a high dose of Pongamia Pinnata extract with antitubercular drugs reduced MDA levels and elevated thiol levels considerably (p˂ 0.05). These biochemical marker levels, however, were not adjusted.
Conclusion: In rats, Pongamia Pinnata encompasses a partial protective effect against the hepatotoxicity caused by antitubercular drugs at high doses.
Keywords: Pongamia Pinnata, isoniazid, rifampin, hepatoprotective