Efficacy of Aspergillus sp in the Production of Protease Enzyme with Different Substrates
D. Manimekalai
Department of Microbiology, A.V.V.M. Sri Pushpam College,(Autonomous), Poondi, Thanjavur, Tamilnadu, (Affiliated to Bharathidasan University, Trichy-24), India.
G. Senthilkumar *
Department of Microbiology, A.V.V.M. Sri Pushpam College,(Autonomous), Poondi, Thanjavur, Tamilnadu, (Affiliated to Bharathidasan University, Trichy-24), India.
V. Ambikapathy
Department of Microbiology, A.V.V.M. Sri Pushpam College,(Autonomous), Poondi, Thanjavur, Tamilnadu, (Affiliated to Bharathidasan University, Trichy-24), India.
A. Panneerselvam
Department of Microbiology, A.V.V.M. Sri Pushpam College,(Autonomous), Poondi, Thanjavur, Tamilnadu, (Affiliated to Bharathidasan University, Trichy-24), India.
*Author to whom correspondence should be addressed.
Abstract
In the present study, the soil samples were collected from marine environment of Arichalmunai, Dhanushkodi, Ramnad District, Tamilnadu ,India. Fungal species were isolated by plating method, in 50% sea water containing potato dextrose agar medium .Totally 16 fungal species were isolated and identified from the soil sample. The production of protease from Aspergillus niger , A.flavus and A. terreus by liquid state fermentation. The production of protease enzyme was optimized by using fermentation medium containing different substrates. The maximum protease production was observed on wheat bran, containing medium. The protease production was maximum in temperature 35ºC were recorded. Wheat bran produced the maximum level protease. The optimization work also carried out. This study revealed that coastal environment provides impressive density of fungi in the East Coast of India and are unexplored for microbial resources can be useful in industry.
Keywords: Marine soil, optimization, substrates, Aspergillus sp, protease enzyme