HPTLC Densitogram Profile of Aqueous Distillate (Arka) from Roots of Boerhaavia diffusa
Pooja . *
School of Pharmaceutical Sciences, IFTM University, Moradabad, India.
V. K. Lal
Lavanya Pharmacy Pvt. Ltd. Chinhat, Lucknow, Uttar Pradesh, India.
A. Verma
School of Pharmaceutical Sciences, IFTM University, Moradabad, India.
*Author to whom correspondence should be addressed.
Abstract
The aqueous distillate (arka) of Boerhaavia diffusa was prepared and investigated qualitatively for TLC. The following work constitutes the enrichment of constituents present in the aqueous distillate (arka) by partition technique using various solvents such as toluene and ethyl acetate in the ratio of (7:3), toluene:acetone:acetic acid (8.5:1:0.5) chloroform:methanol (9:1) Enrichment was determined by Thin Layer Chromatography and HPTLC. Hence it was concluded that aqueous distillate showed the better result for TLC in different solvent systems tried, but best solvent system selected for TLC was toluene: acetone: acetic acid in the ratio of (8: 0.5:1.5) using 5% methanol-sulphuric acid solution as detecting reagent.
Keywords: TLC, toluene, acetone, acetic acid, B. diffusa.