Isolation of Chemical Constituents and Invitro Antidiabetic Activity of Mirabilis Jalapa Flower Extracts
Deepth D. Kodical
QC Analyst, Micro Labs Ltd, Benagluru-560100, Karnataka, India.
Jennifer Fernandes *
Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Mangaluru-575018, Karnataka, India.
Jane Mathew
Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, NITTE (Deemed to be University), Mangaluru-575018, Karnataka, India.
K. Deepthi
Department of Pharmaceutical Chemistry, Srinivas College of Pharmcy,Valchil, Mangaluru-574143, Karnataka, India.
K. Vijay Bhaskar
Department of Pharmaceutical Chemistry, Manipal College of Pharmacy, Manipal Academy of Higher Education, Manipal-576104,Karnataka,India.
*Author to whom correspondence should be addressed.
Abstract
Objectives: The present work was designed to investigate the phytochemical and in vitro screening of the flower extracts of Mirabilis jalapa.
Materials and Methods: Phytochemical constituents were isolated using column chromatography and characterized of the compounds were carried out using IR, 1H NMR, 13C NMR and mass spectroscopy data. In vitro anti-diabetic activity was evaluated by alpha-amylase inhibition assay and Alpha-glucosidase inhibition assay.
Results: Phytochemical investigation of the ethanol extract revealed the presence of a flavonoid Kaempferol and a triterpenoid Lupeol. In vitro investigation indicated that the Mirabilis jalapa flowers extracts has considerable anti-diabetic activity
Conclusion: The anti-diabetic activity may due to the presence of phytoconstituents like flavonoids, triterpenoids, steroids.
Keywords: Mirabilis jalapa, Nyctaginaceae, Kaempferol, Lupeol, anti-diabetic, alpha-amylase, alpha- glucosidase, acarbose