Study of Preventive Effectiveness of Nigella sativa L. against Rat kidney Stones, Generated by Ethylene Glycol
Mohammad Zarei
Department of Nursing, Shirvan Nursing Faculty and Research Center of Natural Products and Medicinal Plants, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Zeynab Rahmani *
Department of Physiology, Shirvan Nursing Faculty, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Javad Rezazadeh
Samen-Al-Aemmeh Hospital, Social Security Organization, Bojnurd, Iran.
Mousa-Al-Reza Hadjzadeh
Department of Physiology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
*Author to whom correspondence should be addressed.
Abstract
Aims: The objective of this research is to study the preventive effectiveness of Nigella sativa L. against rat kidney stones, generated by Ethylene Glycol.
Methodology: In this study, 40 Wistar rats were categorized randomly into four groups of ten. During the research, drinking water labeled group A is mixed with mineral water through utilization of positive control procedures. Into the drinking water labeled group B and other research groups, 1 percent Ethylene Glycol is added through the administration of negative control. In preventive group C, Nigella sativa is added from the first day of the research period and in the treatment of group D. After the 15th day of the research period, 750 mg/kg Nigella sativa powder is administered into the drinking water of rats per day. At the end of the research, kidney tissue samples of rats were stained with haematoxylin and Eosin through the utilization of an optical microscope. Furthermore, serum and urinary samples of rats were analyzed biochemically.
Results: The results indicated that the number of Calcium Oxalate crystals in group B increased in comparison with that of group A. Biochemical analysis of serum and urinary samples indicated a significant increase in the number of Calcium Oxalate crystals of group B in comparison with group A. Furthermore, the analyses depict a unanimous decrease of crystals in all the research groups (except in group C) in comparison with group B.
Conclusion: The findings of this research indicate that Nigella sativa L. does not have any preventive effectiveness against Calcium Oxalate accumulation.
Keywords: Nigella sativa, kidney stone, urinary stone, calcium oxalate, ethylene glycol.