Anticancer Activity of Leaf Hydro Ethanolic Extract of Aegle marmelos in Human Lung Cancer Cell Mediated through Caspase-3 and Caspase-9 mRNA Expression
R. Sukanth
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-77, Tamilnadu, India.
G. Sridevi *
Department of Physiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai-77, Tamilnadu, India.
J. Selvaraj
Department of Biochemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Velappanchavadi, Chennai-600 077, India.
S. Preetha
Department of Physiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai-77, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Aegle marmelos (AE) is a medicinal plant that comes under the rutaceae family and the plant was used in the past for treating many diseases and illness symptoms. The plant has many effects such as anti-diarrhoeal, antimicrobial, antiviral, radioprotective, anticancer, chemopreventive, antipyretic, ulcer healing, antigenotoxic, diuretic, antifertility and anti-inflammatory properties.
Aim: To know the anticancer activity of hydroethanolic leaf extract of Aegle marmelos over lung cancer cells treated with caspase 3 and caspase 9 mRNA expression.
Materials and Methods: The required chemicals were collected mainly from Canada. The lung cancer cells (A549) were collected from NCCS pune and then RNA was extracted from the cells and then the study was conducted after treating it with caspase 3 and caspase 9 mRNA expression. The cells were treated with many dosage of hydroethanolic extract of Aegle marmelos and the cell viability was noted.
Results: The study reported that extract of Aegle marmelos has a great anticancer activity about 1 fold change over rate of 1.7 for cells treated with caspase 3 and a fold change over of 1 in caspase 9 treated lung cancer cells.
Conclusion: The study concluded an innovative finding that the hydroethanolic leaf extract of Aegle marmelos has a great anticancer activity against lung cancer cells treated with caspase 3 and caspase 9 mRNA expression.
Keywords: Aegle marmelos, anticancer activity, caspase expression, hydroethanolic extraction, innovative