Antidiabetic Activity of Different Fractions of Ethanolic Extract of Dactyloctenium aegyptium in Streptozotocin Induced Diabetic Rats
S. Nagarjuna *
Division of Pharmacology, Raghavendra Institute of Pharmaceutical Education and Research, Krishnam Reddy Palli Cross, Chiyyedu, Anantapuramu-515721, Andhra Pradesh, India.
T. E. Gopala Krishna Murthy
Division of Pharmaceutics, Bapatla College of Pharmacy, Bapatla, Guntur-522101, Andhra Pradesh, India.
A. Srinivasa Rao
Division of Pharmacology, Bhaskara Pharmacy College, Moinabad, Hyderabad-501504, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: The present study was aimed to evaluate the antidiabetic effect of n-hexane, Chloroform, Ethyl acetate and Methanolic fractions from ethanolic extract of Dactyloctenium aegyptium in streptozotocin induced diabetic rats.
Study Design: The animals were divided into seven groups and each group consisted of six rats.
Place and Duration of Study: Laboratory of Raghavendra Institute of Pharmaceutical Education& Research (RIPER), Anantapuramu and 35 days.
Methodology: The animals were divided into seven groups and each group consisted of six rats. Group I-Untreated normal rats, Group II-Untreated diabetic rats, Group III-Diabetic rats treated with Gliclazide 4.5 mg/kg, p.o. for 30 days, Group IV- Diabetic rats treated with n- hexane fraction of EDA (NHF) 50 mg/kg, p.o. for 30 days, Group V- Diabetic rats treated with Chloroform fraction of EDA (CF) 50 mg/kg, p.o. for 30 days, Group VI: Diabetic rats treated with Ethyl acetate fraction of EDA (EAF) 50 mg/kg, p.o. for 30 days, Group VII: Diabetic rats treated with Methanolic fraction of EDA (MF) 50 mg/kg, p.o. for 30 days and the serum was separated and used for the estimation of glucose levels. On 31st day of experiment, best fraction was investigated further for its action on insulin, Hb, HbA1c, oxidative parameters, body weight and cell integrity of pancreas.
Results: Results indicated that animals treated with MF shown significant decrease in blood glucose, HbA1c, malondialdehyde levels and significant increase in insulin, Hb, SOD, catalase, reduced glutathione and body weight.
Conclusion: It could be concluded that methanolic fraction of ethanolic extract of Dactyloctenium aegyptium has favourable effect in bringing down the severity of diabetes however necessary studies are required on characterization of active principles.
Keywords: Dactyloctenium aegyptium, streptozotocin, antidiabetic activity, fractions, glucose.