Antimicrobial, Antioxidant and Anticancer Activities of Turnera ulmifolia (Yellow Alder) Callus
K. Kalimuthu *
Department of Botany, Plant Tissue Culture Division, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
V. Chinnadurai
Department of Botany, Plant Tissue Culture Division, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
R. Prabakaran
Department of Botany, PSG College of Arts and Science (Autonomous), Coimbatore- 641014, Tamil Nadu, India.
P. Subramaniam
Government Boys Higher Secondary School, Chinna Thadagam, Coimbatore, India.
Y. Sarmila Juliet
Department of Botany, Plant Tissue Culture Division, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The current work aims to study the effect of ethanolic and methanolic extracts of Turnera ulmifolia callus on antimicrobial, antioxidant and anticancer activities.
Study Design: The Five grams of leaf derived 30 days old callus powder was soaked in 10 ml of ethanol and methanol overnight and then filtered through Whatman filter paper No. 41 along with 2 gms sodium sulphate to remove the sediments and traces of water in the filtrate. Before filing the filter paper along with sodium sulphate is wetted with ethanol. The filtrate was then concentrated by bubbling nitrogen gas in to solution and reduces the volume to one ml.
Place and Duration of Study: Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, Tamil Nadu, India.
Methodology: Ethanolic and methanolic callus extracts were evaluated for antimicrobial, antioxidant (DPPH & FRAP) and anticancer activities (MTT assay).
Results: The ethanolic and methanolic callus extracts of Turnera ulmifolia showed rich sources of antimicrobial, antioxidants, anticancer and can be incorporated into the drug formulations.
Conclusion: This study conformed the use of callus instead of wild plant for herbal medicine. Further studies are needed for isolation and identification of compounds.
Keywords: Antimicrobial, antioxidant, anticancer, Turnera ulmifolia, breast cancer cell.