High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract
Abdullah Alamami
Department of Basic Medical Science, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya.
Fatma Elshibani *
Department of Pharmacognosy, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya.
Salmin Alshalmani
Department of Pharmacognosy, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya.
Mohamed Ali Sharkasi
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya.
Naema Elremali
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benghazi, Benghazi, Libya.
Areej Daboub
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, University of Zawiya, Libya.
*Author to whom correspondence should be addressed.
Abstract
Aims: This research is focused on the in vitro evaluation of Cistus salviifolius L. antimicrobial properties and the determination of the contents of phenols and flavonoids.
Study Design: This research is analytical study aimed to illustrate the antimicrobial properties and to analyze the methanolic extract contents of aerial parts of Cistus salviifolius L. by high-performance liquid chromatography techniques.
Duration: The study was performed within six months in the Faculty of Pharmacy, Benghazy University.
Methodology: Antimicrobial properties was tested against twelve organisms using Kirby-Bauer disk diffusion sensitivity test and the determination of the contents of phenols and flavonoids was evaluated by running high-performance liquid chromatography techniques.
Results: The findings indicated that catechin is the most abundant flavonoid in C. salviifolius, while gallic acid was the major phenol in the methanolic extract of the plant. The results also revealed that the methanol extracts had a significant antimicrobial potential particularly against Bacillus subtilis and Escherichia coli with MIC (0.98 and 0.49) µg/ml respectively, furthermore the extract was effective against Aspergillus fumigatus with MIC 0.98 µg/ml.
Conclusion: C. salviifolius was highly rich with flavonoids and phenols and has a significant antimicrobial effect.
Keywords: C. salviifolius, antimicrobial, HPLC, flavonoids, phenols