A Newly Synthesized Silver Nanoparticles Mediated Phytomedicine from Linum usitatissimum for Dental Caries
P. Bhuvaneshwari
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
P. Vinoba
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
N. Prabhu *
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
M. Vijay Pradhap Singh
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
M. Rajamehala
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
A. Archana
Department of Biotechnology, Vivekanandha College of Engineering for Women, Elayampalayam, Tiruchengode-637 205, Tamilnadu, India.
*Author to whom correspondence should be addressed.
Abstract
The present study deals with the green synthesis of silver nanoparticles using seed extract of Linum usitatissimum (Flaxseed) with ethanol as solvent and examined for anticariogenic activity using disc diffusion method along with antioxidant activity using DPPH scavenging studies. The ethanolic seed extract revealed the presence of saponins, tannins, alkaloids, glycosides, counarins, anthocyanins, carbohydrates, leucoanthocyanin and xanthoproteins. Characterization of synthesized AgNPs were confirmed by FTIR to confirm the functional group involved in reduction of Ag+ ions, SEM to know the morphology of synthesized nanoparticles and XRD to study the crystallographic structure of nanoparticles. The antioxidant activity of synthesized AgNPs showed that the maximum inhibition of 88.88% for the seed-AgNPs and compared with the standard ascorbic acid (90.74%) was observed at concentration (100 µg/ml). The anticariogenic activity of synthesized AgNPs using the seed of Linum usitatissimum showed the maximum zone of inhibition for Streptococcus salivarius with the radius of 12 mm at a higher concentration of 100 µg/ml. Therefore, it is proposed that the synthesized seed-AgNPs played the efficient role against the antioxidant activity and forms the potential source for anticariogenic activity in dental caries application.
Keywords: Linum usitatissimum, antioxidant activity, DPPH scavenging studies, anticariogenic activity, dental caries