Preparation and Characterization of sPLGA Complex Microspheres as a Promising Carrier for Cucurbitacin B
Yi Zhang
Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China and Department of Experimental Teaching, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Yue Liu
Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Hewei Shao
Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Xiaoyan Han *
Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
*Author to whom correspondence should be addressed.
Abstract
The purpose of this research was to develop a new kind of GA-CS/sPLGA complexed Microspheres delivery system for CuB. In this work, star-shaped poly(lactic-co-glycolic acid) (sPLGA-COOH) and chitosan modified with glycyrrhetic acid (GA-CS) were successfully synthesized. The GA-CS/sPLGA complexed Microspheres for CuB was prepared by emulsion evaporation method. Futhermore, CuB-loaded Microspheres formulations were optimized. The release behaviour of CuB-loaded Microspheres in vitro were also examined. It was showed that CuB-loaded amount of Microspheres could reach 58.78%±1.288 by adding GA-CS into the external water phase. The size of corresponding CuB-loaded Microspheres was 370±16.14 nm in diameter, and zeta potential was negative 10.86±0.4790 mV measured by laser light particle size/zeta potential analysis. The results indicated that the complexed GA-CS/sPLGA Microspheres raised the entrapment efficiency for CuB. In vitro drug release demonstrated that complexed GA-CS/sPLGA Microspheres had many advantages such as sustained release and low initial drug burst.
Keywords: Cucurbitacin B, star poly(lactic-co-glycolic acid), complexed microspheres, chitosan modified by glycyrrhetic acid.