Experimental Study of the Effect of Serum Hydrogen Sulfide on the Course of the Inflammatory Process in the Vaginal Wall
Dmytro Grebeniuk
Department of Endoscopic and Cardiovascular Surgery, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Natalia Voloshchuk
Department of Pharmacology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Oleksandr Nazarchuk *
Department of Microbiology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Nataliia Dzis
Department of Obstetrics and Gynecology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Illia Taran
Department of Pharmacology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Elina Slyvka
Department of Obstetrics and Gynecology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Andriy Hryhorenko
Department of Obstetrics and Gynecology, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Mykola Melnychenko
Department of Anaesthesiology and Intensive Care, National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Pavlo Gormash
National Pirogov Memorial Medical University, Vinnytsya, Vinnytsia, Ukraine.
Natalia Hodovan
Communal Non-Profit Enterprise “Vinnytsia Regional Pathological Bureau of Vinnytsia Regional Council”, Vinnytsia, Ukraine.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Various pathological conditions are characterized by the influence of hydrogen sulfide level on the course of the pathological process. This study examines the effect of serum hydrogen sulfide levels on the inflammatory process in the vaginal wall of rats.
Aims: To evaluate the effect of excess and deficiency of serum hydrogen sulfide on the course of the inflammatory process in the vaginal wall of rats.
Methodology: The study was performed on 125 female Wistar rats under 1 year of age and weighing 160.0 to 200.0 grams. All animals were divided into 7 groups: control (intact rats) and 6 experimental groups with different H2S levels and different treatment approaches of inflammation in the vaginal wall. The level of serum hydrogen sulfide was studied and the levels of TNF-α and IL-1β in the tissue homogenate of the vaginal wall were determined. In all experimental groups, the study was performed in dynamics - 10 min, 4, 8 and 24h after simulation of inflammation.
Results: The dynamics of local levels of TNF-α and IL-1β in all groups had a similar trend and was characterized by the rapid development of the inflammatory process from its simulation to 4 hours of study, followed by gradual attenuation of inflammation and almost complete normalization of the studied indicators for 24 hours. Preliminary serial introduction of sodium hydrosulfide, as a donor of hydrogen sulfide, allowed to reduce the degree of manifestation of the inflammatory process and to achieve faster normalization of the studied parameters. At the same time, the artificially created deficiency of serum hydrogen sulfide (previous serial administration of propargylglycine) prolonged the duration and increased the studied indicators of inflammation in the vaginal wall.
Conclusions: The course and intensity of the inflammatory process in the vaginal wall of rats are directly dependent on the background level of serum hydrogen sulfide.
Keywords: Hydrogen sulfide, vaginal wall, inflammation, TNF-α, IL-1β, clindamycin phosphate, rats