Quantitative Analysis of Acteoside in Batankor Syrup and Plantago lanceolata L. Collected from Different Areas of Rwanda by HPLC and UV-Vis Spectrophotometric Methods
Goretti M. Ingabire
National Industrial Research and Development Agency (NIRDA), P.O.Box 273, Kigali, Rwanda.
Andre Ndagijimana
National Industrial Research and Development Agency (NIRDA), P.O.Box 273, Kigali, Rwanda and JSS College of Pharmacy, Rocklands, Post Box No.20, Udhagamandalam 643001, Tamil Nadu, India.
Marie Jeanne Mukazayire
National Industrial Research and Development Agency (NIRDA), P.O.Box 273, Kigali, Rwanda.
George Nyombaire
National Industrial Research and Development Agency (NIRDA), P.O.Box 273, Kigali, Rwanda.
Justin N. Kabera *
National Industrial Research and Development Agency (NIRDA), P.O.Box 273, Kigali, Rwanda.
*Author to whom correspondence should be addressed.
Abstract
A range of biological activities has been found from Plantago lanceolata L. extracts showing anti-inflammatory, analgesic and antioxidant activities. It is in this perspective that the plant was the target of our investigations in order to develop a standardized phytomedicine. Batankor syrup formulated from P. lanceolata L. is used to treat cough and other infections of the respiratory tract. Various active compounds such as flavonoids, glycosides and terpenoids have been identified from Plantago species. The present study was performed to estimate the acteoside content in formulated Bantakor syrup and P. lanceolata leaves collected from four different region of Rwanda. During this investigation, both UV-Vis Spectrophotometric and HPLC methods were developed and validated for determination the content of acteoside in analysts. The chromatographic fingerprints of P. lanceolata extracts represent phyto-equivalence even thought, there are, some differences in their profiles. The retention time of acteoside in the samples was at 6.9 minutes compared to the peak of standard. The plant sample from Rwabuye showed highest yield of acteoside (1.837%) while sample from Ngoma showed less acteoside content (0.594%). The content of acteoside in 1ml of Batankor syrup was found to be 142.19 µg ±0.83. The intraday precision (% RSD) on the basis of content of acteoside was found to be 0.09–0.45 whereas interday precision (% RSD) on the basis of the content were found to be 0.35-0.83, showing the stability of the sample within five days. Also, LOD and LOQ values were 4.26 and 12.90 respectively.
Keywords: Plantago lanceolata, Batankor syrup, acteoside, quantification, chromatographic methods.