Wound healing and Metabolite Profiling in Collagen-Chitosan Biomaterial-treated Chronic Wounds of Hansen’s Disease Patients
Journal of Pharmaceutical Research International,
Aims: Impaired wound healing causes chronic ulcers in Hansen’s disease (HD) patients which are an unrecognized clinical manifestation and requires utmost care and attention for wound management. Collagen and chitosan biopolymers when synergistically combined produce a biologically active biomaterial for wound dressings. Hence, the aim was to prepare a collagen/chitosan (COL/CS) composite and characterize for wound healing potential in HD patients.
Place and Duration of Study: CSIR-Central Leather Research Institute, Sardar Patel Road, Adyar, Chennai 600021, Southern Railway Headquarters Hospital, Constable Road, Ayanavaram, Chennai 600023, and Gremaltes Hospital, India between June 2013 and July 2020.
Methodology: The HD wounds were measured by Planimetry in square cm and were also assessed for morphological structure of epidermis and collagen fiber arrangement by High Resolution- Scanning electron microscopy (HR-SEM). Proton Nuclear magnetic resonance spectroscopy (1H-NMR) for metabolite identification was studied in blood plasma samples of unwounded, untreated and treated HD patients
Results: Size D (wound size on day of discharge) of the wounds were appreciably lower than Size 0 (wound size before biomaterial treatment) demonstrating efficient wound healing by the biomaterial. The morphological structure of the HD wounds showed healthy epidermal layer and thick fibers of collagen matrix in the treated wounds when compared to the controls. Key metabolites of metabolic pathways such as TCA cycle, creatine cycle and protein metabolism were identified by 1H-NMR spectroscopy
Conclusion: The COL/CS wound dressing is a promising biomaterial for management of chronic wounds in Hansen’s disease.
- chronic ulcers
- hansen’s disease
- metabolite profiling
- NMR spectroscopy
- wound healing
How to Cite
Guo S, DiPietro LA. Factors affecting wound healing. Journal of Dental Research. 2010;89(3):219-229.
Mohammadi Z, Zak MS, Majdi H, Mostafavi E, Barati M, Lotfimehr H et al. The effect of chrysin-curcumin-loaded nanofibers on the wound healing process in male rats. Artificial Cells, Nanomedicine and Biotechnology. 2019; 47:1642-1652.
Abd El-Hack ME, El-Saadony MT, Shafi ME, Zabermavi NM, Arif M, Batiha GE et al. Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review. International Journal of Biological Macromolecules. 2020;164: 2726–2744.
Ghomi ER, Khalili S, Khorasani SN, Neisiany RE, Ramakrishna S. Wound dressings: Current Advances and Future directions. Journal of Applied Polymer Science. 2019;47738.
Vojtova L, Pavlinakova, V., Muchova, J. Kacvinska, K, Brtnikova J, Knoz M. et al. Healing and angiogenic properties of collagen/chitosan scaffolds enriched with hyperstable FGF2- STAB® protein: in vitro, ex ovo and in vivo comprehensive evaluation. Biomedicines. 2021;9(6):590.
Zhang MX, Zhao WY, Fang QQ, Wang XF, Chen CY, Shi BH, Zheng B, Wang SJ, Tan WQ, Wu LH. Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays. Journal of Applied Biomaterials and Functional Materials. 2021;19:1-10.
Dorazilova J, Muchova J, Smerkova K, Kociova S, Divis P, Kopel P et al. Synergistic effect of chitosan and selenium nanoparticles on biodegradation and antibacterial properties of collagenous scaffolds designed for infected burn wounds. Nanomaterials. 2020; 10(10):1971.
Peng L, Xiang RC, Jia WW, Dong XX, Wang GE. Preparation and evaluation of porous chitosan/collagen scaffolds for periodontal tissue engineering. Journal of Bioactive and Compatible Polymers. 2006;21:207-220.
Tong C, Hao H, Xia L, Liu J, Ti D, Dong L et al. Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagen-chitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia. Wound Repair and Regeneration. 2016;24:45-56.
Upadya GM, Govindarajan S. Novel treatment for Hard-to-heal chronic plantar ulcers in Hansen’s disease. Journal of Clinical and Diagnostic Research. 2019;13(3):WD01-WD03.
Miyashiro D, Cardona C, Valente NYS, Avancini J, Benard G, Trindade MAB. Ulcers in leprosy patients, an unrecognized clinical manifestation: a report of 8 cases. BMC Infectious Diseases. 2019;19:1013
Upputuri B, Srikantam A, Mamidi RS. Comorbidities associated with non-healing associated with plantar ulcers in leprosy patients. PLOS Neglected Tropical Diseases. 2020;14(6):e0008393
Babu PS, Srinivasan H, Dhandapani BS, Rose C. Collagen-chitosan composite powder as a novel biomaterial for chronic wounds in Hansen disease-A clinical study. Journal of Pharmaceutical Research International. 2021;33(49B): 154-165.
Kim CL, Kim DE. Self-healing characteristics of collagen coatings with respect to surface abrasion. Scientific Reports. 2016; 6:20563.
Tamer TM, Valachova K, Hassan MA, Omer AM, El-Shafeey M, Eldin MSM et al. Chitosan/hyaluronan/edaravone membranes for anti-inflammatory wound dressing: in vitro and in vivo evaluation studies. Materials Science and Engineering: C: Materials for Biological Applications. 2018;90:227-235.
Jayakumar R, Prabaharan M, Sudheeshkumar PT, Nair SV, Tamura H. Biomaterials based on chitin an chitosan in wound dressing applications. Biotechnology Advances 2011;29: 322-337.
Weiss L, Nessler Y, Novelli M, Laheurte P, Grosdidier T. On the use of functionally graded materials to differentiate the effects of surface severe plastic deformation, roughness and chemical composition on cell proliferation. Metals. 2019; 9(12):1344.
Wang K, Qi Z, Pan S, Zheng S, Wang H, Chang YX et al. Preparation, characterization and evaluation of a new film based on chitosan, arginine and gold nanoparticle derivatives for wound- healing efficacy. RSC Advances. 2020; 10:20886.
Sahoo NK, Tejaswini G, Sahu M, Muralikrishna KS. An overview on NMR spectroscopy based metabolomics. International Journal of Pharmaceutical Sciences and Development Research. 2020; 6(1):016-020.
Lindon JC, Nicholson JK, Everett JR. NMR spectroscopy of biofluids. In: Webb GA, editor. Annual reports on NMR spectroscopy. Academic Press; 1999.
Bharti SK, Roy R. Metabolite identification in NMR-based metabolomics. Current Metabolomics 2014; 2:163-173.
Jana SK, Dutta M, Joshi M, Srivastava S, Chakravarty B, Chaudhury K. 1H NMR based targeted metabolite profiling for understanding the complex relationship connecting oxidative stress with endometriosis. Biomed Research International. 2013; 329058
Abstract View: 138 times
PDF Download: 38 times