Hepatoprotective Effect of Papaya Seed Ethanol Extract on Rifampicin Isoniazid-induced Rats
Yessi Sunari Wahfar
Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia.
I. Nyoman Ehrich Lister *
Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia.
Edy Fachrial
Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
Aims: Hepatotoxicity induced by anti-tuberculosis drugs, including rifampicin and isoniazid (AT- DILI, Anti Tuberculosis-Drug Induced Liver Injury), is an adverse reaction followed by significant morbidity. Several in vivo and in vitro research has confirmed that papaya seeds contain various non-essentials, minerals, and fiber. Carica papaya role in disease prevention through modulation of various processes, such as anti-inflammatory, anti-diabetes, immunomodulatory activity, and antioxidant activity, suggests a role in neutralizing free radical generation and ultimately preventing pathogenesis. This study aimed to determine the hepatoprotective effect of ethanol extract of papaya seeds on rifampicin and isoniazid- induced rats.
Study Design: This study is experimental study.
Methodology: The experimental animals in this study were divided into eight groups, including normal group, negative group 1, negative group 2, negative group 3, positive group, treatment group I (papaya seed ethanol extract dose of 100 mg/kgBW), treatment group II (papaya seed ethanol extract dose of 300 mg/kgBW), and treatment group III (papaya seed ethanol extract dose of 500 mg/kgBW), then the rats were dissected, and blood was taken for AST, ALT, ALP, GGT, and Bilirubin level measurements.
Results: The results showed that papaya seed ethanol extract could reduce ALT, AST, ALP, GGT, and Bilirubin levels that were significantly different (P <0.05) than those in the negative control group.
Conclusion: Flavonoid contains in the extract ethanol carica papaya has vita role to prevent the liver toxicity caused by isoniazid and rifampicin.
Keywords: Ripampicin, isoniazid, hepatoprotective