Exploration of Culturable Marine Streptomyces from Southern Coastal Region of India with Antibacterial Properties
Deepika Thandayuthapani
Department of Biotechnology, Bharathidasan University, Tiruchirappalli-620020, Tamil Nadu, India.
Nivetha Chinnappa
Department of Biotechnology, Bharathidasan University, Tiruchirappalli-620020, Tamil Nadu, India.
Arjunan Annavi
Department of Biotechnology, Bharathidasan University, Tiruchirappalli-620020, Tamil Nadu, India.
Muthusevam Manickam *
Department of Biotechnology, Bharathidasan University, Tiruchirappalli-620020, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The Southern coastal region is a geographically positioned area with rich source of microbial diversity, of which producing broad spectrum of bioactive compounds. The marine sediments are collected from the various sites of south east coastal region of Tamil nadu and are processed. Out of 78 isolates, the 4 strains of actinomycetes are showing broad spectrum activity against Klebsiella pneumoniae (MTCC 1687), Proteus vulgaris (MTCC 3160), Salmonella typhi (MTCC 3231), Shigella dysenteriae (MTCC 3642) and Vibrio cholerae (MTCC 3906). The bioactive isolates are proceeded for further morphological features like growth pattern and mycelial coloration, biochemical and polyphasic taxonomical characterisation were documented. Isolates are investigated for abiotic stress condition to study the growth rate of isolates. Finally, the 16S rRNA molecular identification and phylogenetic analysis of the isolates were explored. Further, bioactive isolates may be potential source for discovery of molecules with industrial applications.
Keywords: Chemotaxonomy, phylogenetic analysis, International Streptomyces Project (ISP), MH agar