Phytonutrients and Antimicrobial Activities of Methanolic Extract from Hafr Al-Batin Truffles
Journal of Pharmaceutical Research International,
The desert truffle is a wild mushroom, also referred to as Kamah or Fagaa. Kamah is a rich source of polysaccharides that have medicinal, antitumoral, antibacterial, and immune-stimulant effects. Studies of hypogeous fungi, especially desert truffles, have recently entered traditional studies of epigeous higher Basidiomycetes. Based on the tasty desert truffle Kamah obtained from Hafr Al-Batin Governorate, Saudi Arabia, as a source of potential antimicrobial agents with both the aim of obtaining novel agents toward bacteria and Fungi of clinical significance. We specifically tested the antibacterial and antifungal efficacy of methanol extracts of Kamah against the Gram-negative bacterial pathogens reference strains E. coli ATCC® 8739, P. Aeruginosa ATCC®9027, S. aureus ATCC®6538, Enterococci NCTC®775 and opportunistic fungus C. albicans ATCC®1231.The extract had MIC (minimum inhibitory concentrations) varying from 100 g/ml to 500 g/ml against the pathogens examined. The LC-QTOF-MS (liquid chromatography coupled to quadrupole time of flight mass spectrometry) phytoconstituents assay chromatogram indicated that the methanol extracts of Kamah comprises 264 with retention periods varying from 1.04 to 18.86, which were categorized as unsaturated and saturated natural ingredients sch as aromatic compounds, carboxylic acids, oxygenated hydrocarbons, fatty acids, amino acids, and vitamins).The main compounds were discovered to be 21 with peak areas larger than 2X10-5 and retention periods varying from 2.3 to 9.13.The main known substances with the maximum peaks were adenosine (11.724), phenylalanine (7.711), phenprobamate (7.711), and 5-hydroxytryptophan (5.711). Such preliminary findings, we assume, are encouraging in terms of obtaining a beneficial antibiotic substitute to battle antibiotic-resistant pathogens especially eye infections.
- Hafr Al-Batin
How to Cite
Aldal'in HK, Al-Mazaideh GM, AL-Nadaf AH, Al-Rimawi F, Afaneh AT, Marashdeh A, Abu-Lafi S, Alakhras F, Ayyal-Salman H, Jamhour RMAQ. Phytochemical constituents of securigera securidaca seed extract using. Trop J Nat Prod Res. 2020;4(9):540-544.
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Proc. 2011;89(3):217-33.
Martins S, Amorim ELC, Peixoto Sobrinho TJS, Saraiva AM, Pisciottano MNC, Aguilar CN, Teixeira JA, Mussatto SI. Antibacterial activity of crude methanolic extract and fractions obtained from Larrea tridentata leaves. Ind Crop Prod. 2013;41:306-11.
Wedian F, Al-Qudah MA, Al-Mazaideh GM. Corrosion inhibition of copper by Capparis spinosa L. Extract in Strong Acidic Medium: Experimental and Density Functional Theory. Int J Electrochem. Sci. 2017;12:4664-4676.
Sumayya AR, Srinivasan S, Amatullah N. Screening and biochemical quantification of phytochemicals in fenugreek (Trigonella foenum-graecum). Res J Pharm., Biol Chem Sci. 2012;3(1):165-169.
Patel S. Food, health and agricultural importance of truffles: A review of current scientific literature. Curr Trends Biotechnol Pharm. 2012;6:15-27.
Zambonelli A, Iotti M, Hall I. Current status of truffle cultivation: Recent results and future perspectives. Micol Ital. 2015;44:31-40. DOI: 10.6092/issn.2465-311X/5593
Payen T, Murat C, Bonito G. Truffle phylogenomics: New insights into truffle evolution and truffle lifecycle. Adv Botan Res. Elsevier: Amsterdam, The Netherlands. 2014;70:211-234.
Tzianabos AO. Polysaccharide immunomodulators as theu-rapeutic agents: Structural aspects and biological function. Clin Microbiol Rev. 2000;13:523-533.
Hobbs C. Medicinal value of Lentinus edodes (Berk) Sing (Agaricomycetideae). A literature reviews. Int J Med Mushrooms. 2000;2:287-302.
Hashem A, Alqarawi AA, Shah MA, Wirth S, Egamberdieva D, Tabassum B, Abd_Allah EF. Desert truffles in Saudi Arabia: Diversity, ecology, and conservation. In: Sharma S, Varma A. (eds) Microbial Resource Conservation. Soil Biol. Springer, Cham. 2018;54.
Manzelat SM. Mushrooms in Saudi Arabia (local and imported). Int J Curr Res Biosci. Plant Biol. 2019;6(8):8-12.
Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 2002;60(3):258-74. DOI: 10. 1007/s00253-002-1076-7
Epub 2002 Sep 10. PMID: 12436306.
Al-Qarawi AA, Mridha MAU. Status and need of research on desert truffles in Saudi Arabia. J Pure Appl Microbiol. 2012;6:1051-1062.
El-Enshasy H, Elsayed EA, Aziz R, Wadaan MA. Mushrooms and truffles: Historical biofactories for complementary medicine in Africa and in the Middle East. Evid-Based Compl Alter Med. 2013;2013:1-10.
Schillaci D, Cusimano MG, Cascioferro SM, Stefano VD, Arizza V, Chiaramonte M, Inguglia L, Bawadekji A, Davino S, Gargano ML, Venturella G. Antibacterial activity of desert truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa. I J Med Mushrooms. 2017;19(2):121-125.
DOI: 10.1615/ IntJMed Mushrooms.v19.i2.30
Paul NC, Kim WK, Woo SK, Park MS, Yu SH. Diversity of endophytic fungi associated with Taraxacum coreanum and their antifungal activity. Mycobiology. 2006;34:185-190.
O'Callaghan RJ. The pathogenesis of Staphylococcus aureus eye infections. Pathogens. 2018;7(1):9.
DOI: 10.3390 / pathogens7010009
PMID: 29320451; PMCID: PMC5874735.
Tofighi Z, Molazem M, Doostdar B, Taban P, Shahverdi A, Samadi N, Yassaa N. Antimicrobial activities of three medicinal plants and investigation of flavonoids of tripleurospermum disciforme. Iran J Pharm Res. 2015;14(1):225-231.
Calicchia ML, Wang CIE, Nomura T, Yotsuzuka F, Osato DW. Selective Enumeration of Bifidobacterium bifidum, Enterococcus faecium, and streptomycin-resistant lactobacillus acidophilus from a mixed probiotic product. J Food Prot. 1993;56(11):954-957.
Chung YC, Chan.g C, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extracts from red beam fermentd by Bacillus subtilis MR-K1. J. Agricult Food Chem. 2002;50(8):2454-2458.
Maiti M, Kumar GS. Biophysical aspects and biological implications of the interaction of benzophenanthridine alkaloids with DNA. Biophys Rev. 2009;1(3):119-129.
Jaradat NA, Abualhasan M, Iyad Ali I. Comparison of anti-oxidant activities and exhaustive extraction yields between wild and cultivated Cyclamen persicum, Malva sylvestris and urtica pilulifera leaves. J Appl Pharm Sci. 2015;5(4):101-107.
Murcia MA, Martinez-Tome M, Vera A, Morte A, Gutierrez A, Honrubia M, Jimenez AM. Effect of industrial processing on desert truffles terfezia claveryi Chatin and Picoa juniperi Vittadini: Proximate composition and fatty acids. J Sci Food Agric. 2003;83:535-541.
Janakat SM, Al-Fakhiri SM, Sallal AK. Evaluation of antibacterial activity of aqueous and methanolic extracts of the truffle terfezia claveryi against Pseudomonas aeruginosa. Saudi Med J. 2005;26(6):952-957.
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