A Simple LC-MS/MS Method for Determination of Vemurafenib in Rat Plasma Fed with High Fat Diet
Ayşe Özdemir
Division of Biochemistry, School of Medicine, Uşak University, Uşak, Turkey.
Senem Şanli *
Department of Chemistry, Faculty of Science and Arts, Uşak University, Uşak, Turkey.
Erten Akbel
Department Physiotherapy and Rehabilitation, Faculty of Health Sciences, Uşak University, Uşak, Turkey.
*Author to whom correspondence should be addressed.
Abstract
In this study, high fat diet was fed to rats and the amount of vemurafenib in rat plasma was determined by the developed liquid chromatography with tandem mass spectrometry. The calibration curve was linear between 0.01 and 0.8 µg mL−1 vemurafenib with 0.999 regression coefficient. The limit of detection and quantification of the method are estimated from the signal to noise ratio 3:1 and 10:1, respectively. These are 1.10-4 µg mL−1 for LOD and 4. 10-4 µg mL−1 for LOQ. This method has been found to be reproducible and highly sensitive and provides a combination of faster analysis time and improved limits of detection.
Keywords: Vemurafenib, rat plasma, LC-MS/MS, fat diet