Comparative Evaluation of Phytochemical Constituents by GC-MS and Antitubercular & Antimicrobial Potential of Ceiba pentandra and Parmotrema perlatum against Resistant Strains
K. Varalakshmi Devi
SKU College of Pharmaceutical Sciences, SK University, Anantapuramu, India.
E. Bhargav
Department of Pharmaceutical Chemistry, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu - 515721, Andhra Pradesh, India.
G. Swaruparani
JSS College of Pharmacy, Ooty, Tamilnadu, India.
M. Vijaya Jyothi *
Department of Pharmaceutical Chemistry, Raghavendra Institute of Pharmaceutical Education and Research, K. R. Palli Cross, Chiyyedu Post, Anantapuramu - 515721, Andhra Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Ceiba pentandra and Parmotrema perlatum lichen are two illustrious spices. Apart from their benefit as aromas, These are known for various therapeutic activities. Both are well known as appetizers. Decoctions of theses powders are used to relieve cough, anorexia and helminthiasis etc. Though several studies on antimicrobial activities of these two plants are available, studies on resistant microorganisms and anti tubercular activity are very limited. Hence, the author made an attempt to identify the phytochemical constituents present in methanolic and n-hexane extracts of Ceiba pentandra and Parmotrema perlatum using phytochemical tests and GC-MS method then to evaluate antibacterial activity against 5 resistant microorganisms by microtitre broth dilution method using ciprofloxacillin & streptomycin as reference standards. Methanolic extracts of Parmotrema perlatum and Ceiba pentandra had shown a considerable antibacterial activity than n-hexane extracts and exhibited 90% growth inhibition against H37Rv resistant strain of mycobacterium at 80 & 320 micrograms/ml respectively. N-hexane extracts of both species were found to be poorly effective against H37 Rv.
Keywords: Ceiba pentandra, Parmotrema perlatum, antimicrobial activity, anti tubercular activity, microtitre broth dilution method, micro plate Alamar blue assay