Potential of Ethanol Extracted Secondary Metabolites of Plants from Thal Desert (Pakistan) for In vitro Changes in Haematological Indices
Ghulam Yasin *
Department of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Mubasharah Sabir
Department of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Iqra Anwar
Department of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Adeela Altaf
Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Syeda Amna Batool
Department of Botany, University of Narowal, Narowal, Pakistan.
Ikram Ul Haq
Institute of Biotechnology and Genetic Engineering (IBGE) University of Sindh, Jamshoro, Pakistan.
Ali Noman
Department of Botany, Government College University, Faisalabad, Pakistan.
Khalid Hussain
Department of Botany, University of Gujrat, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Plants are adapted to stressful environments by synthesizing secondary metabolites as antioxidant having either toxicological or medicinal properties. Before utilizations of metabolites one should explore reality about their nature. By testing their in vitro effects on blood, their curing or toxic potential for influence on physiology and health status of an individual can be evaluated. In this experiment, secondary metabolites of some shrubs of Thal desert of Pakistan were extracted in ethanol and used to explore their in vitro effects on some hematological attributes as enlisted in tables. Results were statistically analyzed using Analysis Of Variance. Means of data were compared to control by DMR (Duncan’s multiple range) procedure. Ethanolic extract increased the leucocytes count granulocytes percentage, monocytes lymphocytes MCV, MCH and platelets except extract of Haloxylon stem. HBG were decreased by extract of stems of Leptadenia, Calligonum and Salsola. Leptadenia stem extract also decreased the platelets. Lymphocytes, monocytes, eosinophils and RBC were increased by most of the plant extracts. The practical application of the plant secondary metabolites needs careful further extensive study.
Keywords: Secondary metabolites, potential, ethanolic extracts, Thal desert, haematological indices