Studies on Pancreatic Gene Expression in Diabetic Rats Treated with Moringa oleifera Leaf
Arvin Nwakulite
Department of Medical Laboratory Science, Madonna University, Elele, Rivers State, Nigeria.
Emmanuel Ifeanyi Obeagu *
Department of Medical Laboratory Science, Madonna University, Elele, Rivers State, Nigeria and Department of Medical Laboratory Science ,Imo State University, Owerri, Imo State, Nigeria.
H. U. Nwanjo
Department of Medical Laboratory Science ,Imo State University, Owerri, Imo State, Nigeria.
D. C. Nwosu
Department of Medical Laboratory Science ,Imo State University, Owerri, Imo State, Nigeria.
I. N. Nnatuanya
Department of Medical Laboratory Science, Madonna University, Elele, Rivers State, Nigeria.
C. C. N. Vincent
Department of Nursing Science, Imo State University, Owerri, Imo State, Nigeria.
Chukwudi Ofodile Amaechi
Department of Medical Laboratory Science , Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Anambra State, Nigeria.
Ogochukwu, Mary-Theodora Barbara Ochiabu
Department of Medical Laboratory Science , Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Anambra State, Nigeria.
Adaobi Maryann Ibekwe
Department of Nursing Science, Nnamdi Azikiwe University,Nnewi Campus, Nnewi, Anambra State, Nigeria.
Chukwuma J. Okafor
Department of Pathology and Biochemistry, State University of Zanzibar, Tanzania.
Getrude Uzoma Obeagu
Department of Nursing Science, Ebonyi State University, Abakaliki, Nigeria.
Nkiruka Millicent Amadi
Department of Medical Laboratory Science, Enugu State University of Science and Technology, Enugu State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Moringa oleifera leaf have been used for treatment of diabetes, in this work we studied pancreatic gene expression. The gene expression was done by PCR machine. The plant was harvested from garden within Madonna University and was identified in the Department of Pharmacognosy of the University by Dr P. Osuagwu. Each of the extracts was analyzed for bioactive components using Gas Chromatography–Mass Spectrometry (GC-MS). Male wistar albino rats (n=40) six weeks old weighing 150-250g were purchased from the animal farm of Madonna University Elele. From this work we observed that, there was significant increase in the expression of ADIPOQ gene in the diabetic rats treated with 300mg/kg of M. oleifera leaf (1.52±0.13), when compared with that of the non-diabetic rats (1.00±0.02). From this study, it could be inferred that M. oleifera leaf powder contains polyphenols that inhibit carbohydrate metabolism, reduced blood glucose level, enhance expression ADIPOQ genes.
Keywords: Pancreatic gene expression, diabetic rats, Moringa oleifera leaf