Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme
Angelie Jessica Subbiah
Research Department, Bharath Institute of Higher Education and Research, Chennai-600073, Tamil Nadu, India and Department of Anatomy, KSR Institute of Dental Science and Research, Tiruchengodu -637215 Tamil Nadu, India.
M. Kavimani *
Department of Anatomy, Sree Balaji Medical College, Chromepet, Chennai-600044, Tamil Nadu, India.
Mukilan Ramadoss
Department of Physiology, KSR Institute of Dental Science and Research, Tiruchengodu -637215 Tamil Nadu, India.
Mudiganti Ram Krishna Rao
Department of Industrial Biotechnology, Bharath University, Chennai -620073, Tamil Nadu, India.
K. Prabhu
Department of Anatomy, Sree Balaji Medical College, Chromepet, Chennai-600044, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Many plants provide a rich source of bioactive chemicals, which are free from undesirable side effects and possess powerful pharmacological actions. The present study was carried out to find the antidiabetic effect of Katakakhadiradi kashayam (KKK) by improving the insulin expression and regulating properly the glucose metabolising enzymes. The diabetes was induced in combination with streptozotocin and nicotinamide injection to Wistar rats. Diabetic rats were treated with Katakakhadiradi kashayam orally at doses of 100, 200 and 300 mg/kg/bw for 28 days, and the obtained results of parameters were compared with glibenclamide. The antidiabetic effect of Kashayam was measured by the expression of insulin by immunohistochemistry and restoring the normal clinical values of glucose metabolizing enzymes. The present study specified that hyperglycemia leads to pathological conditions in pancreatic tissue with decreased expression of insulin in β-cells whereas the Katakakhadiradi kashayam normalised the production of insulin. The study found that the antihyperglycemic activity of Katakakhadiradi kashayam L. is mainly due to their ability to restore the function of pancreatic tissues by causing an increase in insulin output and maintaining the glucose metabolising enzymes.
Keywords: Diabetes, hyperglycemia, insulin, immunohistochemistry, Katakakhadiradi kashayam