A New Heterocyclic 1H-perimidine synthesized 2-(2,3-dihydro-1H-périmidin-2yl-)-6-methoxyphenol: Evaluation of Acute Toxicity in Wistar Rat
Tuo Nanou Tiéba
Laboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte-d’Ivoire.
Pacôme Kouadio N’Go *
UFR Des Sciences Biologiques, Université Péléforo Gon Coulibaly de Korhogo, BP 1328 Korhogo, Côte d’Ivoire.
Ballo Daouda
Laboratoire de Chimie Organique Heterocyclique URAC 21, Pôle de Compétence Pharmacochimie, Faculté des Sciences, Université Mohammed V, Rabat, Morocco and Laboratoire de Constitution et Réaction de la Matière (LCRM), UFR Sciences des Structures de la Matière et Technologie, Université Félix Houphouët-Boigny, 22 BP 582 Abidjan, Côte d’Ivoire.
Kangah Niameke Jean Baptiste
Laboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte-d’Ivoire.
Kodjo Charles Guillaume
Laboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte-d’Ivoire.
Yapo Ossey Bernard
Laboratoire Centrale de l’Environnement (LCE), Centre Ivoirien Antipollution (CIAPOL), 20 BP 650 Abidjan 20, Côte d’Ivoire and Laboratoire des Sciences de l’Environnement (LSE), UFR SGE, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte-d’Ivoire.
Ziao Nahossé
Laboratoire de Thermodynamique et de Physico-Chimie du Milieu, UFR SFA, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte-d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Background and Aim: The 1H-perimidne, as novel source carbene ligand, is well known for its anti-fungal, anti-microbial or anti-tumor activities. Here, we aimed to study the acute toxicity in Wistar rat of 2-(2,3-dihydro-1H-perimidin-2-yl)-6-methoxyphenol, a new heterocyclic 1H- perimidine synthetized in our laboratory.
Materials and Methods: Five groups of males Wistar rats were intraperitoneally injected with 7 mg/kg, 40 mg/kg, 90 mg/kg, 130 mg/kg and 150 mg/kg dissolved in dimethyl sulfoxide (DMSO), and followed during 14 days. We noted any clinical signs of acute toxicity as body weight loss, salivation, tremor, convulsion among others, as well as food consumption and water intake level.
Results: The DL50 of the new 2-(2,3-dihydro-1H-perimidin-2-yl)-6-methoxyphenol was estimated to 65 mg/kg. The No Observed Adverse Effect Level (NOAEL) dose was 7 mg/kg because it did not caused mortality, clinical signs of acute toxicity, and not affected feed and water intake behavior. However, significant abnormalities as inflammation and necrosis were observed at doses-effects dependent in liver, when compared to NOAEL dose and vehicle.
Conclusion: The new heterocyclic 2-(2,3-dihydro-1H-perimidin-2-yl)-6-methoxyphenol is considered as high toxicity grade product. From NOAEL dose, subsequent biological, toxicological, pharmacological and neurobehavioral studies are needed before using for clinical trials.
Keywords: Acute toxicity, lethal dose 50, wistar rat, new heterocyclic 1H-perimedine, 2-(2,3-dihydro-1H-perimidin-2-yl)-6-methoxyphenol