Journal of Pharmaceutical Research International

Antidiabetic and anti-inflammatory activity of ethanol and methanol extracts of in vivo and in vitro plants of Ceropegia juncea were evaluated by in vitro methods. Antidiabetic activity was studied using α-amylase inhibitory assay and α-Glycosidase inhibitory assay. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization assay. Results showed that the in vivo and in vitro plant ethanol and methanol extracts exhibited significant activity with α-amylase inhibitory assay and α-Glycosidase inhibitory activity with IC₅₀ values of 434.75±1.40, 578.16±0.09, 555.9±0.28, 748.35±0.58 µg/ml and 752.94±0.82, 797.17±1.02, 877.09±0.74 and 959.86±0.26 µg/ml respectively. The extracts also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and human red blood cell membrane (HRBC) stabilization with the IC₅₀ values of 376±1.21, 372±0.92, 396±0.84, 344±01.08 µg/ml and 376±0.89, 910±1.06, 760±0.56, 810±0.12 µg/ml respectively. Thus, the both in vivo and in vitro plants of C. juncea could potentially be rich sources of natural antidiabetic and anti-inflammatory medicine. From the results, it is concluded that the metabolites present in vitro plant is almost similar to that of in vivo plant. So, in vitro plants can be used for medicinal purpose instead of in vivo plants.