Phytochemical Profiling and Antioxidant Activity of Aqueous Leaf Extract of Vitex negundo
Govindarajan Umamaheswari
Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
Kuppusamy Selvam
*
Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
Palanisamy Prakash
Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Natural products from plants, animals and minerals are the basis for treating human diseases. The present investigation explains that the antioxidant potential of aqueous extract of vitex negundo leaves. Phytochemical constituents, total phenolic and flavanoid content of aqueous analysis and vitro methods such as 1,1-diphenyl-2picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays and GC_MS studies are reported. In addition, TPC and TFC of the extract were evaluated. It was observed that the leaf extract total phenol (14.3 mg) and flavanoids (8.5 mg) Quercetin equivalent (Q/g) high level of Phenolic and flavonoid content that might have accounted for the strong activity observed ABTS 62% of inhibition µg/ mL almost equivalent to that of standard vitamin C and DPPH value of 66.32 µg/ mL almost equivalent to that of standard vitamin C results revealed that leaves of vitex negundo have Since this investigation is a preliminary study, a detailed study of the antioxidant mechanisms of specific phenol components is an absolute necessity. Many phytochemical constituents which may be responsible for many pharmacological activities.
Keywords: DPPH, ABTS, Vitex negundo, phenolic, flavonoid, GC-MS