Effect of Olea europaea L. and Juglans regia L. Extracts on Human Cancer Cell Line Viability with Studying of Hypoglycemic and Antiglycation Properties
Abdulkarim Dakah
*
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Raida Khalil
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Chahed Al Masri
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Marwa Al Matar
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Saba Hameed
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Huzaifa Yousif
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Eman Albsoul
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
Tala Issa
Department of Biotechnology and Genetic Engineering, Philadelphia University, Jordan.
*Author to whom correspondence should be addressed.
Abstract
There is an urgent and continuous need to discover new sources of medicinal plants to obtain useful compounds with health properties. So the purpose of this study was to investigate activity of Olea europaea L and Juglans regia leaves extract on Hela cell line viability, antidiabetic and Antiglycation. The aqueous extracts were obtained from leaves. Alloxan 180 mg /kg body was used to induce diabetes. Mice with blood glucose level of ≥200 mg/dl were considered as diabetic and were received 10 mg/kg of body weight of Extracts. For Antiglycation, SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) were prepared for the appearance of high molecular weight products. Hela cell line were cultured in RPMI-1640 medium for MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay analysis. The results showed that the mean of glucose levels decreasing in mice that treated with 10 mg/Kg extracts of O. europaea and J. regia. Also SDS-PAGE showed that extracts of J. regia were better than of O. europaea in inhibition of glycation induced protein cross-linking at all studied concentrations. MTT assay showed that the Cytotoxicity was increased with increasing the doses of extract and the cytotoxic effects of J.regia extract were higher than O.europaea at all concentrations. This study showed promising results.
Keywords: Olea europaea L, Juglans regia L, hypoglycemic, antiglycation, Hela cell line, MTT.