Recent Advances and Developments in Hyphenated Techniques and their Applications
Jero Victor Wilson
Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
L. V. Karthikeyan
Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
Shubham Kumar Parida
Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
Bikash Chandra Nath
Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
M. R. Jeyaprakash *
Department of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Background: A hyphenated technique is blend or coupling of two distinctive analytical methods like chromatographic and spectral methods with appropriate interface.
Objective: This article describes about the various hyphenated techniques, a brief note on their instrumentation and working principles that are used in the current setup of industries. Like-wise their remarkable improvement and efficiency over the past decade. The techniques like single quadrupole inductively coupled mass spectrometry (ICP-Q-MS), ICP- Triple quadrupole mass spectrometry (ICP-QQQ), Liquid chromatography-Two -dimensional Gas chromatography mass spectrometry (LC-GCXGC-MS/MS), Two dimensional liquid chromatography (2D-LC), Fourier transform near infrared spectroscopy (FT-NIR) ,etc. are considered as recent improvements in this trend.
Conclusion: This development in the hyphenated techniques supports in different scientific fields, biomedical research, food and drug analysis. In addition to this improvement produced remarkable dimensional changes in the natural product analysis and elemental specifications.
Keywords: LC-MS, FT-IR, chromatographic techniques, mass spectrometry.