The Effects of Lamotrigine on Fetus Resorption and Histologic Changes in Cranium of Fetus of Albino Mice
Nadeemuddin Shaikh
Department of Anatomy, GMMMC, SMBBMU Larkana, Pakistan.
Abdullah Khilji
Department of Anatomy, Khairpur Medical College, PUMHS, Shaheed Benazirabad, Pakistan.
Syed Faizan Ali Rizvi *
Department of Anatomy, GMMMC, SMBBMU Larkana, Pakistan.
Bushra Begum Ramejo
Department of Obstetrics and Gynecology, Khairpur Medical College, PUMHS, Shaheed Benazirabad, Pakistan.
Hafsa Shafqat
Department of Anatomy, Ziauddin University Karachi, Pakistan.
Noor Ahmed Shaikh
Department of Paeds, GMMMC Sukkur, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Background: Lamotrigine is a member of antiepileptic drugs, it belongs to the sodium channel blocking agent’s class and it is pregnancy category C drug. While its teratogenic effects are not hidden by the doctors but it is the preferred drug being prescribed in pregnancy. The current study aims to investigate the effects of fetus resorption and histologic changes in cranium of fetus of albino mice and to compare their weight changes due to lamotrigine therapy.
Methodology: It was an experimental animal study conducted in collaboration of anatomy and surgery department at animal house of University of Lahore in 2019. The duration of study was 25 days, twenty-four albino mice (12 males and 12 females) were placed in conventional cages in pairs for mating purpose. Female mice Group A, controls in which normal saline was administered intraperitoneally on 10th day of gestation and Group B, in which lamotrigine was given intraperitoneally on the 10th day at the dose of 10 mg/kg. Maternal health was monitored daily during the intervention. Body weight, food and water consumption, and changes in general health, behavior, activity and any sign of toxicity were checked daily. After 18th day the pregnant mice were sacrificed under euthanasia and fetuses were removed and histologic assessment was carried out.
Results: Weight of mice treated with lamotrigine decreased significantly (p-value=0.03) and fetus resorptions were also more (p-value=0.013) in Group B. Histologic assessment reveled that there were cleft of lip and palate in group B.
Conclusion: Lamotrigine increased the fetal resorption and decrease the weight and seemed to be responsible for inducing cleft of lip and palate at 10mg/kg dose in albino mice.
Keywords: Lamotrigine, fetus resorption, cranial abnormalities, albino mice.