Exploration of the Chemical Potential and Antioxidant Activity of Some Plants Used in the Treatment of Male Infertility in Southern Benin
Jean Robert Klotoé *
Unité de Recherche en Microbiologie Appliquée et Pharmacologie des Substances Naturelles, Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin and Ecole Normale Supérieure de Natitingou, Université Nationale des Sciences, Technologie, Ingénierie et Mathématiques, BP72 Natitingou, Bénin.
Eric Agbodjento
Unité de Recherche en Microbiologie Appliquée et Pharmacologie des Substances Naturelles, Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin.
Victorien Tamègnon Dougnon
Unité de Recherche en Microbiologie Appliquée et Pharmacologie des Substances Naturelles, Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin.
Mahudro Yovo
Laboratoire d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin.
Téniola Isabelle Sacramento
Ecole de Gestion et d’exploration des Systèmes d’élevages, Université Nationale d’Agriculture (EGESE/UNA) - Kétou, Bénin.
Esther Déguénon
Unité de Recherche en Microbiologie Appliquée et Pharmacologie des Substances Naturelles, Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin.
Jacques Tossou Dougnon
Unité de Recherche en Microbiologie Appliquée et Pharmacologie des Substances Naturelles, Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey Calavi, Université d’Abomey-Calavi, 01BP2009 Cotonou, Bénin.
Jean Marc Atègbo
Faculté des Sciences et Techniques, Laboratoire de Physiopathologie Moléculaire et Toxicologie, Université d’Abomey-Calavi, 01BP526 Cotonou, Bénin.
*Author to whom correspondence should be addressed.
Abstract
Antioxidants are a family of substances that can neutralize free radicals and prevent and/or treat diseases associated with oxidative stress such as male’s infertility. Medicinal plants are one of the main sources of antioxidants.
Aim: This work was aimed at evaluating the chemical and antioxidant potential of different extracts from some plants used in traditional Beninese medicine for the treatment of male infertility.
Materials and Methods: The study was carried out on aqueous, hydro-ethanolic and ethanolic extracts from of the roots of Gardenia ternifolia (G. ternifolia), the whole plant of Cassytha filiformis (C. filiformis), the leaves of Rourea coccinea (R. coccinea) and the seed of Garcinia kola (G .kola). Quantification of the total polyphenols and flavonoids content of these extracts was evaluated respectively by the method using Folin Ciocalteu and the method using Aluminum trichloride. The antioxidant activity of the extracts was evaluated by molecular spectrophotometry using the free radical scavenging of DPPH and FRAP methods.
Results: The results obtained indicated a variation of total polyphenols and flavonoids content according to the type of extract. Hydro-ethanolic extract of the various plants studied has a high polyphenols and flavonoids content. In variable proportions, all the extracts tested reduced the DPPH radical and ferric iron, reflecting their antioxidant potential. The best antioxidant activity has been obtained with the hydro-ethanolic extracts.
Conclusion: This study showed that all the plant's extracts studied have antioxidant activity that varies with the type of extract. However, the hydro-ethanolic extractions showed the best antioxidant activities. The data obtained in the present study justified the use of these plants in management of pathologies involving oxidative stress.
Keywords: Antioxidant activity, medicinal plant, male’s infertility, Benin.